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Due to its lipophilicity, Pepaxto is rapidly taken up by multiple myeloma cells, where it is rapidly hydrolyzed by peptidases to release the hydrophilic alkylating agent

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For TRPV1 immunostaining, the skin segments of mice were first incubated with a primary rabbit polyclonal TRPV1 antibody (dilution factor: 1:100) overnight at 4–8 °C and then washed with PBS to remove the primary antibody