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Zebrafish Embryos were cultivated in an incubator at a suitable temperature (29°C) using embryo medium (EM) containing 5 mM NaCl, 0.33 mM CaCl 2 , 0.17 mM KCl, and 0.33 mM MgCl 2 , with a 14 h light/10 h dark cycle ( 2.3 Auditory brainstem response Auditory brainstem responses (ABR) were recorded for three groups of mice at the age of 6, 10 and 14 weeks using BioSigRZ software from Tucker Davis Technologies (TDT, FL, USA)

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